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Epimedin C inhibited M2 <t>macrophage</t> markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; <t>BMDM,</t> <t>bone</t> <t>marrow-derived</t> macrophage.
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primary bone marrow derived macrophages bmdm  (Jackson Laboratory)
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Epimedin C inhibited M2 <t>macrophage</t> markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; <t>BMDM,</t> <t>bone</t> <t>marrow-derived</t> macrophage.
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Epimedin C inhibited M2 <t>macrophage</t> markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; <t>BMDM,</t> <t>bone</t> <t>marrow-derived</t> macrophage.
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Epimedin C inhibited M2 <t>macrophage</t> markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; <t>BMDM,</t> <t>bone</t> <t>marrow-derived</t> macrophage.
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Epimedin C inhibited M2 <t>macrophage</t> markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; <t>BMDM,</t> <t>bone</t> <t>marrow-derived</t> macrophage.
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murine bone marrow derived macrophages bmdms  (Jackson Laboratory)
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Epimedin C inhibited M2 <t>macrophage</t> markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; <t>BMDM,</t> <t>bone</t> <t>marrow-derived</t> macrophage.
Murine Bone Marrow Derived Macrophages Bmdms, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bone+marrow-derived+macrophages+(bmdms)/bone+derived+macrophages+marrow/pmc12758812-79-0-17
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murine bone marrow derived macrophages bmdms - by Bioz Stars, 2026-10
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primary rat bone marrow derived macrophages bmdms 256 primary bmdms  (Charles River Laboratories)
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Epimedin C inhibited M2 <t>macrophage</t> markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; <t>BMDM,</t> <t>bone</t> <t>marrow-derived</t> macrophage.
Primary Rat Bone Marrow Derived Macrophages Bmdms 256 Primary Bmdms, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Epimedin C inhibited M2 macrophage markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; BMDM, bone marrow-derived macrophage.

Journal: Scientific Reports

Article Title: Epimedin C attenuates airway inflammation and remodeling in Asthma by intervening M2 macrophage polarization via modulating the PI3K/Akt/mTOR signaling pathway

doi: 10.1038/s41598-026-42160-4

Figure Lengend Snippet: Epimedin C inhibited M2 macrophage markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; BMDM, bone marrow-derived macrophage.

Article Snippet: Mouse bone marrow-derived macrophages (BMDM) purchased from Procell (CP-M141; Wuhan, China) were cultured in BMDM complete medium (CM-M141; Procell) containing 10% foetal bovine serum (FBS; Hyclone).

Techniques: In Vitro, Immunofluorescence, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Control, Derivative Assay